Within the same image, what does the fluorescent green color represent?

Within the same image, what does the fluorescent green color represent?

• A. Epithelial nuclei
• B. Total bacteria (FITC-Eub) adhered to mucosa
• C. Fusobacterium
• D. Bacteroides

Answer: (B)

The green fluorescence comes from a universal bacterial probe that binds to conserved regions of bacterial rRNA. With FITC-labeled Eub (EUB338), this probe lights up essentially all bacteria, so the green signal marks total bacteria adhered to the mucosal surface. Epithelial nuclei are stained separately with a different dye and would appear as a different color, not green. Fusobacterium and Bacteroides are specific taxa; they would contribute to the green signal only as part of the overall bacterial presence detected by this universal probe, rather than being distinguished on their own.

The green fluorescence comes from a universal bacterial probe that binds to conserved regions of bacterial rRNA. With FITC-labeled Eub (EUB338), this probe lights up essentially all bacteria, so the green signal marks total bacteria adhered to the mucosal surface. Epithelial nuclei are stained separately with a different dye and would appear as a different color, not green. Fusobacterium and Bacteroides are specific taxa; they would contribute to the green signal only as part of the overall bacterial presence detected by this universal probe, rather than being distinguished on their own.